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KMID : 0384119870070010107
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Korean Journal of Clinical Pathology
1987 Volume.7 No. 1 p.107 ~ p.113
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Evaluation of Methods for Detection of Terminal Deoxynucleotidyl Transferase in Human Hematologic Malignancies
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Abstract
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Terminal deoxinucleotidyl transferase (TdT) antigen assay was done in 34 human specimens of bone marrow, peripheral blood and lymph node by using enzyme im￢munoassay (EIA) (Abbott Lab,.), and immunofluorescence (IF) (Bethesda Research Lab.) .
For EIA, specimens yielding >5Ong/10$ cells in peripheral blood or > lO0.ng/10$ cells in bone marrow, and for IF, >5% in peripheral blood or ´10% in bone marrow were defined as positive. Judgement of positive or negative by both tests correlated cor￢rectly in 94% of the cases studied. When the EIA is considered the standard method, the sensitivity and specificity of IF method were about 94,°0. A good positive cor￢relation between TdT-EIA antigen concentration and percentage of TdT-IF positive cells was obtained (r = 0. 80).
Both EIA and IF methods are suitable for routine use in clinical laboratory. EIA is appropriate for batch analysis of - 10 or more specimens collected and has the ad￢vantage of quantitative. determinaton. IF method is easier and economical to process single specimen at the time of request and can be used as a reliable test for leuke￢mia-lymphoma marker study in a laboratory with good experience of immunofluore￢scence study.
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